A 1-Up Mushroom is a rare item occasionally found in some levels. Once collected, it gives the player an extra life. If the player has 99 lives, 1-Up Mushrooms give 20 rupees when collected.
When the player loses all lives, they receive a Game Over.
Occasionally, the 1-Up Mushroom takes on a different appearance depending on the character and circumstances.
- If Mario or Luigi are the current character, the 1-Up Mushroom has its default appearance.
- If Arthur is the playable character, it will take the form of an extra life icon from his series.
- If Link is the current character, it used to take the form of the Triforce but as of v0.5, Link's 1-Ups changed to the Link dolls from Zelda 2.
- When found in Sonic-style monitors, it will bear the face of the current character.
- If Classic Mario or Classic Luigi is the player character, its form will be that of the 8-bit 1-Up from Super Mario Bros.
- When playing as a Mega Man character (Roll, Proto Man, Mega Man, Vile, Zero), its form will be that of the character's head.
- When playing as Wario, the 1-Ups appear as 1-Up Hearts from the Wario series.
- When playing as Waluigi, the 1-Ups appear as a green eggplant.
Also, the sound effect of the 1-Up also depends on what character the player has selected. The sound effect is also heard when the player saves the game.
- If the player has selected Mario, the 1-Up sound effect from Super Mario World and Super Mario All-Stars is played.
- If the player has selected Luigi, the 1-Up sound effect from Super Mario World 2: Yoshi's Island is played.
- If the player has selected Wario, the 1-Up sound effect from Super Mario Land is played.
- If the player has selected Waluigi, the 1-Up sound effect from Super Mario Land 2: 6 Golden Coins is played
- If the player has selected Sonic or Tails, the 1-Up sound effect from the Sega Master System version of Sonic The Hedgehog is played.
- If the player has selected one of the Mega Man characters, the 1-Up sound effect from Mega Man 1 is played.
- If the player has selected Link, the sound effect for achieving an item from The Legend Of Zelda is played.
Standard | Super Mushroom | Fire Flower | Raccoon Leaf | Hammer Bros. Suit | Tanooki Suit | Blue Shell | |
Crossover | Sci-Fi Suit | Mega Man Suit | Soldier Suit | |
Other | 1-Up Mushroom | 3-Up Moon | Poison Mushroom | Starman | Lil Reaper | Stomp Boots | Life Energy | |